Kyriakou Z, Corral-Baques MI, Amat A, Coussios CC. HIFU-induced cavitation and heating in ex vivo porcine subcutaneous fat. Ultrasound in Medicine and Biology. 2011;37(4):568-79.
The present study is motivated by the fact that there are no published studies quantifying cavitation activity and heating induced by ultrasound in adipose tissue and that there are currently no reliable techniques for monitoring successful deposition of ultrasound energy in fat in real time. High-intensity focused ultrasound (HIFU) exposures were performed in excised porcine fat at four different frequencies (0.5, 1.1, 1.6 and 3.4 MHz) over a range of pressure amplitudes and exposure durations. The transmission losses arising from reflection at the skin interface and attenuation through skin and fat were quantified at all frequencies using an embedded needle hydrophone. A 15 MHz passive cavitation detector (PCD) coaxial to the HIFU transducer was used to capture acoustic emissions emanating from the focus during HIFU exposures, while the focal temperature rise was measured using minimally invasive needle thermocouples. Repeatable temperature rises in excess of 10°C could be readily instigated across all four frequencies for acoustic intensities (Ispta) in excess of 50 W/cm(2) within the first 2 s of exposure. Even though cavitation could not be initiated at 1.1, 1.6 and 3.4 MHz over the in situ peak rarefactional (p(-)) pressure range 0-3 MPa explored in the present study, inertial cavitation activity was always initiated at 0.5 MHz for pressures greater than 1.6 MPa (p(-)) and was found to enhance focal heat deposition. A good correlation was identified between the energy of broadband emissions detected by the PCD and the focal temperature rise at 0.5 MHz, particularly for short 2 s exposures, which could be exploited as a tool for noninvasive monitoring of successful treatment delivery.
Corral-Baqués MI, Rivera MM, Rigau T, Rodríguez-Gil JE, Rigau J. The Effect of Low Level Laser Irradiation on Dog Sperm Motility Is Dependent on Laser Output Power. Lasers in Medical Science. 2009;24(5):703-713.
Biological tissues respond to low-level laser irradiation and so do dog spermatozoa. Among the main parameters to be considered when a biological tissue is irradiated is the output power. We have studied the effects on sperm motility of 655 nm continuous wave diode laser irradiation at different output powers with 3.34 J (5.97 J/cm(2)). The second fraction of fresh dog sperm was divided into five groups: control, and four to be irradiated with an average output power of 6.8 mW, 15.4 mW, 33.1 mW and 49.7 mW, respectively. At 0 min and 45 min after irradiation, pictures were taken and a computer aided sperm analysis (CASA) performed to analyse different motility parameters. The results showed that different output powers affected dog semen motility parameters differently. The highest output power showed the most intense effects. Significant changes in the structure of the motile sperm subpopulation were linked to the different output powers used.
Costa JM, Cáncer L, Corral-Baqués MI, Amat A. Tratamiento de 15 casos de dolor crónico con un equipo de fotopuntura por diodos emisores de luz roja (617 nm). Revista Internacional de Acupuntura 2008 Vol 2 N. 1:63-66
En el presente estudio se presentan los primeros casos tratados con un nuevo equipo de fotopuntura por diodos emisores de luz (light emitting diode, LED) diseñado por SORISA. El equipo consiste en un sistema de LED, con 10 canales que emiten luz roja de 617nm. Método: se han tratado 15 pacientes con dolor crónico de diferente localización: cervicobraquialgia (3 casos), lumbalgia/ciática (4 casos), gonalgia (3 casos), cefalea (2 casos), talalgia (1 caso), epicondilitis (1 caso) y neuralgia del trigémino (1 caso). Para caracterizar el nivel de dolor, se ha usado la Escala Categórica de Dolor (no dolor , leve [1-3], moderado [4-6] y grave [7-10]). Sólo se trataron pacientes con dolor grave (7-10). Los pacientes recibieron 2 sesiones por semana de 25 min cada una, y se realizaron un total de 5 a 8 sesiones. Los parámetros de tratamiento fueron los siguientes: potencia de cada salida de 10mW, emisión pulsada a 60 Hz, y dosis total por punto tratado de 7,5 J. Para caracterizar la respuesta al tratamiento, los resultados se han clasificado como: “sin resultado”, cuando no hubo cambio en el grado de dolor; “ligero”, cuando el dolor sólo disminuyó en una categoría; “bueno”, cuando el dolor disminuyó en 2 categorías y “muy bueno” en la remisión completa del dolor (sin dolor). Resultados: un caso “muy bueno”, 11 casos “bueno” y 3 casos con un resultado “ligero”; no hubo ningún caso “sin resultado”. Estos primeros casos permiten anticipar que el equipo de fotopuntura LED puede ser una buena alternativa a la acupuntura clásica en el tratamiento del dolor crónico, y se considera que aún es necesaria una mayor experimentación al respecto.
Costa JM, Corral-Baques MI, Amat A. Acupuncture. a first approach on pain relief using a 617 nm LED device. Progress in biomedical optics and imaging. ISSN 1605-7422. 2007, Vol. 8, nº5, [Note(s): 64280ª. 1-64280ª.6]
In this study, a preliminary approach for pain relief using a novel pulsated LED device was conducted. 12 patients were treated with a Photopuncture device designed by SORISA, which consisted in a 10-channel LED system at 617 nm. 15 patients with different pain localizations were treated: cervicobrachialgia (3 cases), lumbago/sciatica (4 cases), gonalgia (3 cases), cephalalgia (2 cases), talalgia (1 case), epicondylitis (1 case) and trigeminal neuralgia (1 case). To characterize the pain level, the Categorical Pain Scale (none (0), mild (1-3), moderate (4-6) and severe (7-10) was used). Just patients with severe pain (7-10) were treated. Patients were treated twice a week for 25 minutes; 5 to 8 were given at the following treatment parameters: 10 mW per channel pulsed at 60 Hz with a 50% duty cycle. The total dose for point was 7.5 J. To characterize the response to the treatment, the results were classified as: “no result”, no changes in pain degree; “poor”, pain decreased one category; “good”, pain decreased two categories; “very good”, complete healing (no pain). The results were: 1 case with “very good” result; 11 cases with “goo” result, 3 cases with “poo” result; and 0 cases with “no result”. We conclude that the Photopuncture LED device may be a good alternative to classical Acupuncture in pain relief, although further experimentation is required.
Corral-Baques MI, Rigau T, Rivera M, Rodriguez-Gil JE, Rigau J. Effect of 655-nm laser at different powers on dog sperm motility parameter. Biophotonics and New Therapy Frontiers. Vol. 7, No. 35 ISSN 1605-7422 (2006)
Introduction: One of the most appreciated features of the sperm is its motility, which depends on a big energy consumption despite differences among species. Laser acts direct or indirectly on mitochondria increasing ATP production. Materail and method: By means of a Computer Aided Sperm Analysis (CASA) we have studied the effects of a 655 nm continuous wave diode laser irradiation at different power outputs with a dose of 3.34 J on sperm motility. After an eosine-nigrosine stain to establish its quality, the second fraction of fresh beagle dog sperm was divided into 5 groups, 1 control and four to be irradiated respectively with an average output power of 6.84 mW, 15.43 mW, 33.05 mW and 49.66 mW. At times 0 and 45 minutes from irradiation pictures were taken and analysed with the Sperm class Analyzer SCA2002 programme. The motility parameters of 4987 spermatozoa studied were: curvilinear velocity (VCL), progressive celocity (VSL), straightness (STR), wobble (WOB), average path velocity (VAP), linearity (LIN), mean amplitude of lateral head displacement (ALHmed), beat cross frequency (BCF) and the total motility (MT). At time 15 minutes after irradiation a hypoosmotic swelling test (HOST) was done. Results: several motility parameters that affect the overall motile sperm subpopulations structure have been changed by different output powers of a 655 nm diode laser irradiation, and prevents the decrease of the sperm motility properties along time.
Corral-Baques MI, Rigau T, Rivera M, Rodriguez-Gil JE, Rigau J. Effect of 655 nm diode laser on dog sperm motility. Lasers in Medical Science. 2005;20(1):28-34
Sperm motility depends on energy consumption. Low-level laser irradiation increases denosine triphosphate (ATP) production and energy supply to the cell. The aim of this study is to analyse whether the irradiation affects the parameters that characterise dog sperm motility. Fresh dog sperm samples were divided into four groups and irradiated with a 655-nm continuous-wave diode laser with varying doses: 0 (control), 4, 6 and 10 J/cm(2). At 0, 15 and 45 min following irradiation, pictures were taken of all the groups in order to study motility with computer-aided sperm analysis (CASA). Functional tests were also performed. Average path velocity (VAP), linear coefficient (Lin) and beat cross frequency (BCF) were statistically and significantly different when compared to the control. The functional tests also showed a significant difference. At these parameters, the 655-nm continuous-wave diode laser improves the speed and linear coefficient of the sperm.
(Guest Editorial) Corral-Baques M-I, Rigau J. About time to get to speak the same language. Proc. 4th Congress of the World Association for Laser Therapy, Tokyo, Japan, June 27-30. 2002; page 123.